Epicardial Pacemaker Leads

Epicardial pacemaker leads are insulated electrical wires placed on the outer surface of the heart to deliver pacing when conventional transvenous leads are unsuitable or inaccessible. Their electrode contacts the epicardium and myocardium, sensing intrinsic cardiac activity and transmitting timed electrical impulses from an implanted pulse generator to initiate contraction. Clinicians commonly implant these leads during cardiac surgery, particularly in children with congenital heart disease, patients with abnormal venous anatomy, or those at risk of infection from intravascular hardware. Epicardial pacing provides an important long-term or temporary alternative, although lead performance and surgical placement influence durability and clinical outcomes.

Epicardial Pacemaker Leads - Related Videos

Research

JoVE Journal - Developmental Biology

Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration

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Cited by 9 •

2016

Here, we describe methods for isolating primary mouse epicardial cells by an outgrowth culture assay and assessing the functional migration of epicardial-derived cells (EPDC) using an ex vivo heart culture system. These protocols are suitable for identifying genetic and chemical modulators of epicardial epithelial-to-mesenchymal transition (EMT) and motility.

In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart

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Cited by 13 •

2016

The epicardium is an essential source of multipotent cardiovascular progenitor cells and paracrine factors that are required for cardiovascular development and regeneration. We describe here a method to culture mouse embryonic epicardial cells.

High-Resolution Endocardial and Epicardial Optical Mapping in a Sheep Model of Stretch-Induced Atrial Fibrillation

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Cited by 20 •

2011

This report provides a detailed description of the methodology and results of simultaneous endocardial and epicardial optical mapping of electrical excitation in the intact left atrium of a Langendorff-perfused sheep heart during stretch-induced atrial fibrillation.

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

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Cited by 13 •

2015

This protocol describes how to produce functional sinus nodal tissue from murine pluripotent stem cells (PSC). T-Box3 (TBX3) overexpression plus cardiac Myosin-heavy-chain (Myh6) promoter antibiotic selection leads to highly pure pacemaker cell aggregates. These “Induced-sinoatrial-bodies” (“iSABs”) contain over 80% pacemaker cells, show highly increased beating rates and are able to pace myocardium ex vivo.

Education

JoVE Science Education - Environmental Sciences

Lead Analysis of Soil Using Atomic Absorption Spectroscopy

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2023

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Lead occurs naturally in soil, in levels ranging from 10-50 ppm. However, with the widespread use of lead in paint and gasoline in addition to contamination by industry, urban soils often have concentrations of lead significantly greater than background levels – up to 10,000 ppm in some places. Ongoing problems arise from the fact that lead does not biodegrade, and instead remains in the soil. Serious health risks...

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