Fetal Liver Injection
Fetal Liver Injection - Related Videos
05:15
Portal Vein Injection: A Method to Study Cancer Metastasis to the Liver
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This video describes a surgical procedure to deliver mammary tumor cells to the murine liver via portal vein injection. This model permits the investigation of late stages of liver metastasis.
09:24
Decellularization and Recellularization of Whole Livers
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Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
14:19
Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction
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Cited by 11 •
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We describe examination of fetal cardiac function with contemporary functional fetal echocardiography and fetoplacental Doppler ultrasound using the VisualSonics VEVO 2100 microultrasound in a surgically induced model of intrauterine fetal growth restriction in a rabbit.
08:04
Murine Fetal Echocardiography
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Cited by 7 •
2013
Fetal and perinatal death is a common feature when studying genetic alterations affecting cardiac development. High-frequency ultrasound imaging has improved 2-D resolution and can provide excellent information on early cardiac development and is an ideal method to detect the impact on cardiac structure and function prior to death.
FAQs
The fetal liver provides both a vascular setting and a site of active blood formation, so delivered material can reach developing hepatocytes and blood-forming cells. This combination makes the organ useful for examining how cells, genes, or therapeutic agents interact with immature liver and hematopoietic tissues. The resulting exposure helps investigators assess tissue targeting and biological feasibility before birth.
Studies can focus on developing hepatocytes, the liver’s functional cells, as well as blood-forming cells located in the fetal liver. Examining these populations helps distinguish whether an intervention primarily affects liver development, prenatal blood formation, or both. This cellular context is important when evaluating therapies intended to correct disorders involving hepatic or hematopoietic function.
Prenatal transplantation studies may seek donor-cell engraftment before the fetal immune system fully matures. Engraftment refers to the establishment of transplanted cells within the recipient’s tissues. Delivering cells during this developmental window allows researchers to investigate whether early exposure supports donor-cell persistence and informs the long-term therapeutic potential of transplantation approaches.
The access approach determines how material is placed into the developing liver. Image guidance can support visualization during delivery, whereas microsurgical access provides a direct operative route. In either case, accurate placement is central to exposing the intended vascular and hematopoietic environment. Investigators can then assess whether the technique achieves the desired tissue targeting and feasibility.
A typical research workflow identifies the material to be delivered, selects an access strategy, places it within the developing fetal liver, and evaluates the resulting biological response. The material may consist of cells, genes, or therapeutic agents. Follow-up assessment can address tissue targeting, safety, treatment feasibility, donor-cell engraftment, and longer-term therapeutic potential.
This approach supports studies of fetal development and prenatal treatment design. Investigators can use it to evaluate gene delivery, cell-based therapies, and transplantation strategies while examining how effectively the intervention reaches relevant liver or blood-forming cells. Outcomes may clarify whether a treatment is feasible, whether it appears safe, and whether prenatal delivery could provide durable therapeutic value.