Intestinal Inflammation
Intestinal Inflammation - Related Videos
08:43
Investigating Intestinal Inflammation in DSS-induced Model of IBD
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Cited by 607 •
2012
Experimental models of inflammatory bowel disease have allowed us to examine the complex innate and adaptive immune responses associated with pathogenesis. Using histological scoring, quantification of pro-inflammatory cytokines and myeloperoxidase activity, one can begin to assess these responses seen in inflammatory bowel disease.
09:44
Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation
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Cited by 32 •
2012
Postoperative ileus (POI) is a complication of abdominal surgery leading to increased morbidity and a prolonged hospital stay. Because prophylactic or therapeutic strategies are lacking intensified research is necessary. Therefore we established a standardized and feasible mouse model to investigate the pathophysiology of POI and to study potential therapeutic options.
04:06
Using Fluorescence-Mediated Tomography to Assess Murine Intestinal Inflammation
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2025
This video demonstrates a technique for assessing intestinal macrophage infiltration in a murine model of colitis using fluorescence-mediated tomography (FMT). Upon inducing colitis in a mouse via dextran sodium sulfate (DSS), the animal is injected with antibodies that recognize the F4/80 glycoprotein marker on infiltrated macrophages in the colon. The mouse is then imaged in an FMT device where the fluorescence and reflectance signals are reconstructed into a three-dimensional image to...
07:05
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
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Cited by 2 •
2017
Target-specific probes represent an innovative tool for analyzing molecular mechanisms, such as protein expression in various types of disease (e.g., inflammation, infection, and tumorigenesis). In this study, we describe a quantitative three-dimensional tomographic assessment of intestinal macrophage infiltration in a murine model of colitis using F4/80-specific fluorescence-mediated tomography.
07:38
Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
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Cited by 18 •
2016
Accurate assessment of anti-inflammatory effects is of utmost importance for the evaluation of potential new drugs for the treatment of inflammatory bowel disease. Digital holographic microscopy provides assessment of inflammation in murine and human colonic tissue samples as well as automated multimodal evaluation of epithelial wound healing in vitro.
FAQs
Epithelial barrier disruption can alter interactions between the intestinal lining and gut microorganisms while also promoting immune activation. This combination helps explain how a local disturbance develops into a broader inflammatory response. In medicine, examining barrier integrity therefore helps connect tissue injury with downstream immune recruitment and potential impairment of intestinal function.
Innate immune cells provide an early response, whereas adaptive immune cells contribute to a more developed immune reaction. Their activation is part of the signaling network that can sustain intestinal inflammation when the process persists. Distinguishing these contributions helps explain why research may seek treatments that selectively reduce harmful immune activity rather than broadly suppress all defenses.
Cytokines act as signaling molecules that amplify communication during intestinal inflammation. Their activity is linked with increased vascular permeability, which can facilitate recruitment of additional leukocytes. That recruitment connects molecular signaling to cellular accumulation in affected tissue. Understanding this sequence helps investigators interpret how an initially local response may become more extensive when inflammatory signaling continues.
Studying the linked changes in barrier integrity, microorganism interactions, immune-cell activation, and cytokine signaling can identify features relevant to diagnosis and biomarker development. The value of this approach is that it connects biological processes with intestinal injury and dysfunction, helping medicine characterize disease activity and evaluate targets for future intervention.
Persistent intestinal inflammation can damage tissue and contribute to conditions including inflammatory bowel disease. As inflammation continues, it may impair absorption and other aspects of intestinal function. This distinction matters clinically because an immune response that may support repair when acute can become harmful when chronic, linking duration with different outcomes.
Treatments can pursue two complementary goals identified in the mechanism: restoring epithelial barrier integrity or selectively reducing harmful immune activity. These approaches target different points in the process rather than treating intestinal dysfunction as a single problem. Their relevance follows from the contribution of barrier disruption and immune signaling to ongoing tissue damage.