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Chapters
- 00:05Title
- 01:33Collection of Colonic Tissue Samples and Assessment of Colitis Severity
- 03:58Sample Preparation for Myeloperoxidase (MPO) Assay
- 05:09Quantification of Inflammatory Markers
- 06:01Evaluating the Severity of Acute Colitis in DSS-treated Animals
- 08:23Conclusion
Experimental models of inflammatory bowel disease have allowed us to examine the complex innate and adaptive immune responses associated with pathogenesis. Using histological scoring, quantification of pro-inflammatory cytokines and myeloperoxidase activity, one can begin to assess these responses seen in inflammatory bowel disease.
Tags
Intestinal InflammationDSS-induced ModelIBDUlcerative ColitisCrohn’s DiseasePathogenesisGeneticEnvironmental ComponentsAnimal ModelsColonic InflammationDextran Sulphate SodiumColitis-inducing ChemicalsAcute ColitisChronic ColitisWeight LossLoose StoolDiarrheaRectal BleedingHistological AnalysisPro-inflammatory Cytokines
