Murine Aorta Isolation
Murine Aorta Isolation - Related Videos
15:15
Flow Cytometry Analysis of Immune Cells Within Murine Aortas
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Cited by 87 •
2011
This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.
03:13
Porcine Aorta Isolation: A Surgical Procedure to Excise the Thoracic Aorta From Miniature Pigs for Downstream Analysis
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2025
In this video, we demonstrate a procedure for the surgical removal of the aorta from miniature pigs. Endothelial cells harvested from these isolated aortae can be further cultured in in vitro conditions to study coagulation and immune responses.
09:20
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
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Cited by 8 •
2017
This article provides detailed methodology to identify and quantify functional T lymphocyte subsets present within murine kidney, aorta and lymph nodes by intracellular staining and flow cytometry. The model of angiotensin II induced hypertension was chosen to explain, step-by-step, the procedures and fundamental principles of flow cytometry and intracellular staining.
07:25
Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease
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Cited by 16 •
2014
Pathology of the aorta can lead to severe morbidity and mortality, therefore research of disease progression and potential therapies is warranted. Here, we present a protocol to isolate and excise the murine aorta to aid researchers in their investigation of cardiovascular disease.
08:24
Isolation of Murine Coronary Vascular Smooth Muscle Cells
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Cited by 11 •
2016
The purpose of this protocol is to demonstrate the isolation and culture techniques of murine primary vascular smooth muscle cells (VSMCs) from the coronary circulation. Once VSMCs have been isolated, they can be used for many standard culture techniques.
FAQs
Preservation maintains the tissue features that researchers need to examine after dissection. Damage to the aortic wall or disruption of its cellular architecture could compromise subsequent histological or molecular analysis. Careful isolation therefore supports more reliable assessment of vascular structure and disease-related changes in the recovered specimen.
These surrounding materials can interfere with examination of the aortic wall and reduce the consistency of downstream processing. Their careful separation leaves the vessel more suitable for mounting, culture, fixation, or molecular and histological analysis. Removing them while protecting the vessel helps researchers focus measurements on the aorta rather than on attached or residual material.
An ex vivo preparation provides a controlled tissue model in which the isolated vessel can be mounted, cultured, fixed, or processed according to the study objective. This flexibility allows investigators to examine vascular structure and function and to evaluate disease mechanisms or drug responses without limiting analysis to observations made in the intact animal.
The workflow begins with removing the aorta from the mouse and then carefully separating it from surrounding connective tissue, blood, and branching vessels. The recovered vessel must retain its aortic wall and cellular architecture. After dissection, researchers select an appropriate downstream preparation, such as mounting, culture, fixation, or processing for molecular or histological studies.
The intended analysis determines the next handling step. Researchers may mount the vessel, maintain it in culture, fix it for preservation, or process it for molecular or histological studies. These options make the same isolated tissue adaptable to structural examination, cellular assessment, or investigation of changes associated with vascular disease and treatment responses.
The technique supports investigation of atherosclerosis, aneurysm development, inflammation, and responses to drugs. By providing access to isolated vascular tissue, it helps researchers examine structural and functional features linked to these conditions in a controlled model. Findings can contribute to understanding cardiovascular disease mechanisms and evaluating potential therapeutic approaches.