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Flow Cytometry Analysis of Immune Cells Within Murine Aortas
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

DOI:
10.3791/2848-v

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15:15 min

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July 01, 2011

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, , ,
1Deptartment of Microbiology and Molecular Cell Biology,Eastern Virginia Medical School, 2Division of Inflammation Biology,LaJolla Institute for Allergy and Immunology

Chapters

  • 00:05Title
  • 01:39Isolation of Murine Aortas and Adventitia
  • 03:51Preparation of Aortic and Adventitial Single Cell Suspensions
  • 06:15Flow Cytometry Staining
  • 09:42Representative Flow Cytometric Analyses of Murine Aortic and Adventitial Infiltrating Leukocytes

Summary

Automatic Translation

Automatic Translation

This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.

Tags

Flow CytometryImmune CellsMurine AortasAtherosclerosisChronic Inflammatory ProcessPlaquesFoam CellsImmune ResponseInnate ArmAdaptive ArmMacrophagesDendritic CellsT LymphocytesB LymphocytesAortic AdventitiaAtherogenesisQuantitative DetectionRT-PCRImmunohistochemical MethodsFlow Cytometry Analysis

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