Pulmonary Artery Endothelial Cells
Pulmonary Artery Endothelial Cells - Related Videos
10:46
Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
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Cited by 89 •
2010
Here, we describe a protocol for isolation and culture of murine pulmonary endothelial cells. This method comprises mechanic and enzymatic lung tissue dissociation as well as a 2-step purification process using anti-PECAM-1 and anti-ICAM-2 antibodies conjugated to magnetic beads, which produces a pure endothelial cell population of mostly microvascular origin.
08:17
Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery
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Cited by 8 •
2017
The following protocol describes the methodology for the acquisition and analysis of echocardiographic images used to obtain the Left Atrial Volume (LAV), Aorta (Ao) diameter, and Pulmonary Artery (PA) diameter in mice. This technique is a non-invasive, non-terminal procedure that allows assessment of the cardiopulmonary function.
08:02
Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
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Cited by 16 •
2013
We have developed a novel and reproducible technique to isolate primary cultures of pulmonary artery smooth muscle cells (PASMC) from mice as young as P7, thereby allowing better study of the signaling pathways involved in neonatal smooth muscle cell contraction and relaxation.
03:14
Obtaining Cytotoxic Amyloids Following Bacterial Infection of Rat Pulmonary Endothelial Cells
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2025
Source: Balczon, R., et al. Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa. J. Vis. Exp. (2018)This video demonstrates the procedure for generating cytotoxic supernatant from pulmonary endothelial cells following infection with Pseudomonas aeruginosa. It outlines how bacterial effector proteins disrupt host cell structure and induce the release of cytotoxic amyloid oligomers into the supernatant.
08:55
In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
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Cited by 17 •
2013
The endothelial glycocalyx/endothelial surface layer is ideally studied using intravital microscopy. Intravital microscopy is technically challenging in a moving organ such as the lung. We demonstrate how simultaneous brightfield and fluorescent microscopy may be used to estimate endothelial surface layer thickness in a freely-moving in vivo mouse lung.
FAQs
Blood-flow sensing allows these cells to respond to conditions within the pulmonary arteries and adjust signals affecting the vessel wall. This response is relevant because endothelial behavior can influence vascular permeability and communication with smooth-muscle cells. Studying flow-related responses helps researchers examine how altered vascular conditions may contribute to impaired pulmonary blood flow and pulmonary vascular remodeling.
Pulmonary artery endothelial cells integrate oxygen-related signals with inflammatory stimuli, changing how they communicate with surrounding vascular and circulating cells. These inputs can affect the selective barrier between blood and the vessel wall, as well as interactions with immune cells. Examining these responses helps clarify how environmental and inflammatory stress may contribute to vascular injury.
Signaling molecules released by pulmonary artery endothelial cells can influence smooth-muscle contraction, linking endothelial activity to the diameter and functional state of the vessel. When endothelial signaling becomes abnormal, this communication may support changes associated with pulmonary vascular remodeling. This mechanism is therefore important for investigating how vascular regulation becomes disrupted in pulmonary hypertension.
Cultured pulmonary artery endothelial cells provide an experimental system for examining endothelial behavior under controlled research conditions. Investigators can use them to study disease mechanisms, evaluate drug responses, and explore therapeutic strategies intended to preserve pulmonary vascular function. Their use connects cellular observations with broader questions about vascular injury, remodeling, and impaired blood flow.
Drug-response studies can reveal how candidate treatments affect pulmonary artery endothelial behavior and whether they support preservation of pulmonary vascular function. These experiments also help connect a treatment response with disease mechanisms involving permeability, signaling, or cellular interactions. Such findings can guide research on therapies for conditions characterized by pulmonary vascular dysfunction.
They are relevant because endothelial dysfunction is linked in the overview to abnormal pulmonary vascular remodeling and impaired blood flow, both central concerns in pulmonary hypertension research. Cell-based studies allow investigators to examine these processes alongside vascular injury and treatment responses. This provides a focused medical context for testing mechanisms and therapies that may preserve pulmonary vascular function.