A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:25Pulmonary Artery (PA) Isolation from Neonatal Mice
- 03:27Culturing Pulmonary Artery Smooth Muscle Cells (PASMC)
- 06:30Results: Pulmonary Artery Smooth Muscle Cells (PASMC) Expression
- 07:32Conclusion
We have developed a novel and reproducible technique to isolate primary cultures of pulmonary artery smooth muscle cells (PASMC) from mice as young as P7, thereby allowing better study of the signaling pathways involved in neonatal smooth muscle cell contraction and relaxation.
