Submucosal Saline Injection

Submucosal saline injection is an endoscopic technique that lifts gastrointestinal mucosa by placing sterile saline beneath a lesion, helping clinicians assess and treat abnormal tissue safely. During injection, saline enters the submucosal layer and temporarily separates the mucosa from the muscularis propria, creating a fluid cushion between the target tissue and deeper bowel-wall structures. This elevation can facilitate endoscopic mucosal resection by improving lesion capture and providing a clearer working plane, while helping limit inadvertent injury to the muscle layer. The technique is therefore useful in the management of selected polyps, adenomas, and other superficial gastrointestinal lesions.

Submucosal Saline Injection - Related Videos

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JoVE EoE - Neuropathology

A Hypertonic Saline Injection Technique for Inducing Glaucoma in Rats

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2025

This video demonstrates glaucoma induction in an anesthetized rat by injecting hypertonic saline into the episcleral vein. The saline enters the trabecular meshwork via Schlemm's canal, causing tissue scarring, which blocks aqueous humor outflow and raises intraocular pressure. The elevated pressure damages retinal ganglion cells, leading to glaucoma.

The Pocket-Creation Procedure of Endoscopic Submucosal Dissection for Large Rectal Laterally Spreading Tumors

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Cited by 1 •

2026

This study details a standardized PCM-ESD protocol for resecting large rectal LSTs, aiming to enhance dissection speed and prevent transmural perforation via submucosal tunneling.

A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance

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Cited by 6 •

2018

We developed a new ex vivo model that applies constant tension to the porcine gastric specimen. This development made it possible to evaluate the performance (the height and duration of the submucosal elevation) of various SIMs accurately. The detailed setup methodology of this new model is explained.

Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea

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Cited by 17 •

2012

Here we demonstrate our protocol for isolation of basal and submucosal gland duct cells from mouse tracheas. We also demonstrate the method of injecting stem cells into the dorsal mouse fat pad to create an in vivo model of submucosal gland regeneration.

ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection

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Cited by 14 •

2017

This study reports a successful method of endoscopical adipose tissue-derived stromal cell (ADSC)-sheet transplantation for esophageal stricture prevention after an extended endoscopic submucosal dissection (ESD) in a swine model.

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