Tumor Bioluminescence
Tumor Bioluminescence - Related Videos
03:46
Bioluminescent Imaging of Breast Tumor Progression in a Female Mouse Model
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2025
Source: Marina Arantes Radicchi1, Victor Carlos Mello1, Victória Paz Machado1, Márcia Cristina Oliveira da Rocha1, Jaqueline Vaz de Oliveira1, Luana Cristina Camargo1,2, Luís Alexandre Muehlmann1, Ricardo Bentes Azevedo1, Sônia Nair Bao1, João Paulo Figueiró Longo11Institute of Biological Sciences, University of Brasília, 2Department of Basic Psychological Processes, Institute of Psychology, University of BrasíliaThis video demonstrates the procedure for tracking tumor progression in vivo by...
02:08
Bioluminescence Imaging to Assess Tumor Growth in an Organotypic Mouse Brain Slice
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2025
Source: Ciraku, L., et. al. An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth. J. Vis. Exp. (2021).This video demonstrates the imaging of luciferase-expressing tumor cells in an organotypic mouse brain slice. Luciferin is added to the medium, and the plate is placed inside the chamber of the in vivo imaging system. Parameters for image capture are adjusted, and the tumor's shape and size are defined. The bioluminescent signals emitted by the tumor...
07:12
Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models
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Cited by 29 •
2016
Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models.
11:09
Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging
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Cited by 104 •
2010
Luciferase-modified human brain tumor xenografts can be established intracranially in athymic mice, with subsequent monitoring of tumor growth and response to therapy using bioluminescence imaging. In combination with survival analysis, bioluminescence monitoring is an essential research tool for pre-clinical testing of therapies being considered for treating brain tumors.
11:02
In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model
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Cited by 16 •
2011
Bioluminescence imaging of hypoxia inducible factor-1α activity is applied to monitor intracranial tumor hypoxia development in a breast cancer brain metastasis mouse model.
FAQs
Luciferase expression provides the molecular link between tumor cells and the optical readout. Once the substrate, such as luciferin, is administered, luciferase catalyzes a light-producing reaction. The resulting photons travel through surrounding tissue and are captured by a sensitive camera, allowing researchers to associate detected signal with engineered cancer cells.
Changes in signal intensity can serve as a longitudinal indicator of tumor behavior. An increasing or decreasing signal may correspond to changes in tumor burden, while the location or spread of signal can provide information about distribution. Repeated measurements therefore help track progression or treatment response without requiring tissue collection at every time point.
Engineering supplies the enzyme required for the light-producing reaction, creating a measurable connection between labeled cancer cells and the camera signal. Without luciferase expression in the tumor cells, administration of the substrate would not produce the same cell-associated optical readout. This design enables researchers to follow those cells during experiments.
Researchers first establish tumor cells that express luciferase in a living experimental model. They then administer the appropriate substrate, such as luciferin, and use a sensitive camera to detect emitted photons through surrounding tissue. Repeating this sequence over time produces measurements that can be compared across tumor progression or treatment studies.
The approach permits noninvasive observation of cancer-related changes in living experimental models, reducing the need for repeated tissue collection. Because measurements can be obtained over time, researchers can follow tumor progression, metastatic distribution, and response to therapy. This supports longitudinal assessment of therapeutic efficacy while preserving the model for subsequent observations.
Researchers can compare bioluminescent measurements over time to assess whether tumor-associated signal changes after treatment. A change in intensity may indicate altered tumor burden, while a change in distribution may reflect altered spread. The method therefore provides a noninvasive readout for studying therapeutic efficacy alongside cancer progression and metastasis in preclinical models.