Adult Mouse
Adult Mouse - Related Videos
09:00
Olfactory Behavioral Testing in the Adult Mouse
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Cited by 68 •
2009
Fundamental, yet unique properties of the rodent olfactory system have led to its increasing study among biologists. A relatively simple assessment of its function is then also needed. Here we describe sensitive tests for the characterization of mouse olfactory sensitivity and preference.
08:42
Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes
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Cited by 35 •
2016
This protocol describes a step-by-step method for the reproducible isolation and long-term culture of adult mouse cardiomyocytes with high yield, purity, and viability.
10:51
Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
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Cited by 88 •
2017
Epidermal keratinocytes form a functional skin barrier and are positioned at the front line of host defense against external environmental insults. Here we describe methods for isolation and primary culture of epidermal keratinocytes from neonatal and adult mouse skin, and induction of terminal differentiation and UVB-triggered inflammatory response from keratinocytes.
06:42
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis
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Cited by 29 •
2011
In this article, we present a simple, practical technique for cerebrovascular casting that is easy to perform and can be utilized to image the vascular tree of the adult mouse brain.
12:49
Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes
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Cited by 35 •
2013
Here we describe the isolation of adult mouse cardiomyoctyes using a Langendorff perfusion system. The resulting cells are Ca2+-tolerant, electrically quiescent and can be cultured and transfected with adeno- or lentiviruses to manipulate gene expression. Their functionality can also be analyzed using the MMSYS system and patch clamp techniques.
FAQs
Adult mouse studies are especially useful when researchers need to examine neural circuits after they have developed. This makes it possible to investigate established patterns of sensory processing, motor control, and learning rather than focusing on early nervous-system formation. Comparisons with embryonic or juvenile animals therefore help separate developmental effects from mechanisms operating in a mature brain.
The model supports a linked analysis of neural activity and behavior. Genetic approaches or targeted neural manipulation can alter or identify particular biological components, while electrophysiology and imaging provide measurements of neural activity. Behavioral assays then relate those measurements to functions such as sensation, movement, or learning, allowing researchers to connect cellular-level changes with whole-animal outcomes.
Studying neural processes in an intact organism preserves interactions among neurons, glia, and other systems that may be lost when experiments examine neural components apart from the animal. This broader context matters when a neural change affects physiology or behavior through multiple biological pathways, and it helps researchers interpret findings in relation to whole-organism function.
Researchers may combine genetic approaches and targeted neural manipulation with electrophysiology or imaging, then assess behavior and analyze tissue. Each method contributes a different level of evidence: manipulation examines the role of selected neural factors, activity measurements track associated function, behavioral assays reveal consequences, and tissue analysis helps examine biological changes after the experiment.
In neuroscience, adult mouse experiments can address sensory processing, motor control, learning, and age-related changes. The selected behavioral assay focuses on the function under study, while neural recordings or imaging can relate performance to cellular activity. This combination helps distinguish an observed behavioral change from a corresponding alteration in neural function.
Their value extends beyond describing normal brain function. Researchers can use the mature nervous system to investigate disease mechanisms and evaluate therapeutic strategies while observing effects on physiology, behavior, and neural activity. Age-related changes are also accessible in this framework, making the model relevant when disease or treatment outcomes involve an established nervous system.
Tissue analysis provides a complementary endpoint to live measurements and behavior. After neural activity and performance have been examined, researchers can use this approach to assess biological changes associated with the experiment and relate them to the manipulated or recorded system. Including this endpoint helps connect observations made during behavior with underlying cellular or tissue-level findings.