Anterograde Tracing
Anterograde Tracing - Related Videos
11:26
Anterograde Amnesia
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2023
Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California Anterograde amnesia is the loss of the ability to form new memories. This can be distinguished from retrograde amnesia, which is the loss of old memories. Anterograde amnesia can result from damage to structures in the brain that are involved in the formation of new memories. Patients who have damage to the structures of the medial temporal lobe, including the hippocampus, amygdala, and the surrounding...
15:31
Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
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Cited by 16 •
2013
Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.
07:02
Trace Fear Conditioning in Mice
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Cited by 32 •
2014
In the following experiment we describe a protocol for trace fear conditioning in mice. This type of associative memory includes a trace period that separates the neutral stimulus and the unconditioned stimulus.
06:39
Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat
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2018
Here, we present a refined protocol to effectively reveal biotinylated dextran amine (BDA) labeling with a fluorescent staining method through a reciprocal neural pathway. It is suitable for analyzing the fine structure of BDA labeling and distinguishing it from other neural elements under a confocal laser scanning microscope.
11:03
Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
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Cited by 5 •
2014
Capitalizing on a binary genetic strategy we provide a detailed protocol for neural circuit tracing in mice that express complementary transsynaptic tracers after Cre-mediated recombination. Because cell-specific tracer production is genetically encoded, our experimental approach is suitable to study the formation and maturation of neural circuitry during murine embryonic brain development at a single cell resolution.
FAQs
The tracer’s movement follows the pathway from neuronal cell bodies through axons toward presynaptic terminals. This direction allows researchers to associate labeled fibers and terminal fields with the neurons that originate the projection. Because the signal follows axonal routes, the resulting pattern provides structural information about how one defined brain area connects with its target regions.
A defined introduction site establishes the starting population of neurons being examined. Tracer uptake at that location lets researchers follow projections arising from that brain area rather than viewing an undifferentiated pattern across the nervous system. The selected site therefore determines which pathway relationships can be visualized and compared with other regions or experimental conditions.
Labeled fibers show the routes taken by axons, while terminal fields indicate where those projections end near their presynaptic targets. Examining both features helps distinguish a pathway’s course from its destination. This combination supports analysis of projection patterns and strengthens interpretations linking circuit structure with functions such as sensory processing, motor control, or behavior.
After the tracer has moved through the axonal pathway, researchers detect labeled fibers and terminal fields with microscopy or other imaging methods. These approaches make the spatial distribution of the signal visible within the nervous system. The resulting images can then be used to examine projection patterns, compare brain regions, and relate anatomical organization to circuit function.
A typical workflow begins by introducing a tracer into a selected brain area. Neurons at that site take up the tracer and transport it along their axons toward presynaptic terminals. Researchers then use microscopy or another imaging method to detect labeled fibers and terminal fields, producing a map of the projection pattern for analysis.
Researchers apply the method when they need to examine how circuit structure relates to function across brain regions or experimental conditions. It can support investigations of sensory processing, motor control, behavior, and neurological disease. Comparing the resulting projection patterns helps identify similarities or differences in connectivity associated with regions, conditions, or disease-related changes.