Epimark 5-hmc Kit
Epimark 5-hmc Kit - Related Videos
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
2011
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
04:41
Magnetic-Activated Cell Sorting: A Method to Isolate c-Kit Positive Cells
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2023
This video describes the technique of isolating c-kit positive cells from the bone marrow of a mouse. In the protocol, we will isolate c-kit positive cells from a transgenic mouse to validate c-Fos and Dusp1 alone or together as drug targets in leukemia.
01:04
5-Number Summary
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2023
In a dataset, the 5-number summary includes the minimum data value, the data value of the first quartile, the median data value or data value of the second quartile, the data value of the third quartile, and the maximum data value. These 5 data values can be visualized as a box and whisker plot. In a box plot, the minimum and maximum data values represent the lower and upper whiskers in the graph, and the median is designated as the center of the box in the chart. The first quartile and third...
01:23
The 5 c's of Pricing
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2024
The 5 Cs of pricing provide a comprehensive framework for strategic pricing decisions. They include: Company Objectives: The company's goals are pivotal in determining pricing. Whether the aim is to maximize profit, increase market share, or survive in a competitive market, it will directly influence the pricing strategy. Cost: It involves calculating the total cost of producing a product or service, including fixed and variable costs. Pricing must cover these costs and provide a reasonable...
09:00
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Cited by 15 •
2009
The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.
FAQs
Glucose addition is the assay’s discrimination step. T4 phage β-glucosyltransferase specifically adds glucose to existing 5-hydroxymethylcytosine residues, creating a modified DNA state that can be evaluated during restriction-enzyme digestion. Quantifying the sequences that remain protected after digestion provides a basis for distinguishing hydroxymethylated sites from unmodified sites.
Restriction digestion converts the chemical labeling difference into a measurable DNA difference. After β-glucosyltransferase treatment, sequences containing labeled 5-hmC can show protection relative to unmodified sequences, and quantitative analysis of the protected fraction reflects hydroxymethylation at the examined sites. This links molecular recognition to an interpretable assay signal rather than relying only on chemical detection.
Global analysis summarizes hydroxymethylation across genomic DNA, whereas locus-specific analysis focuses on selected genomic sequences. Using either scale, the kit can reveal whether 5-hmC changes broadly or at particular loci. This distinction helps connect overall epigenetic remodeling with changes near genes or genomic regions relevant to neuronal regulation and disease-associated processes.
The workflow begins with genomic DNA, followed by treatment with T4 phage β-glucosyltransferase to add glucose to 5-hmC. The treated DNA then undergoes restriction-enzyme digestion, and the protected sequences are quantified. Comparing the resulting signal with the digestion-based distinction between protected and unmodified DNA supports measurement of hydroxymethylation patterns.
The assay provides quantitative information about protected DNA sequences, which can be used to estimate 5-hmC patterns in genomic DNA. Depending on the selected analysis, the outcome may describe global hydroxymethylation or signal from particular loci. These measurements support comparisons among samples and help identify epigenetic changes associated with biological state or disease-related conditions.
5-hmC is especially abundant and dynamically regulated in the nervous system, making its distribution relevant to neuronal biology. The kit supports investigations of neuronal development, brain aging, disease-associated epigenetic changes, and gene regulation by measuring hydroxymethylation across genomic DNA or at selected loci. Results can therefore connect DNA modification patterns with changing neural states.