Fluorescence Microscopy Observation
Fluorescence Microscopy Observation - Related Videos
09:22
Introduction to Fluorescence Microscopy
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2023
Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light wavelengths by a fluorescent molecule known as a fluorophore. Fluorescence microscopy is accomplished in conjunction with the basic light microscope by the addition of a powerful light source, specialized filters, and a means of fluorescently...
03:15
Fluorescence Microscopy of Neutrophil Extracellular Traps or NETs: A Technique to Observe Adhesive Interaction Between Cancer Cells and NETs
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2023
This video demonstrates the fluorescent staining of neutrophil extracellular traps (NETs), the extracellular DNA components expelled by neutrophils. This technique enables the visualization of adhesive interaction between cancer cells and NETs.
07:28
Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
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Cited by 33 •
2017
Understanding the function of essential processes in bacteria is challenging. Fluorescence microscopy with target-specific dyes can provide key insights into microbial cell growth and cell cycle progression. Here, Agrobacterium tumefaciens is used as a model bacterium to highlight methods for live cell imaging for characterization of essential processes.
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
09:02
Visualizing Bacteria in Nematodes using Fluorescent Microscopy
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Cited by 20 •
2012
To study the mutualism between Xenorhabdus bacteria and Steinernema nematodes, methods were developed to monitor bacterial presence and location within nematodes. The experimental approach, which can be applied to other systems, entails engineering bacteria to express the green fluorescent protein and visualizing, using fluorescence microscopy bacteria within the transparent nematode.
FAQs
The optical system directs excitation light toward labeled material, while filters separate the emitted, longer-wavelength fluorescence from the illumination signal. This separation reduces interference from the excitation beam and makes labeled structures appear with strong contrast against surrounding regions. As a result, researchers can examine the spatial distribution of selected molecules or cellular features within neuronal samples.
These labels determine what biological feature becomes visible. Fluorophore-linked antibodies can identify particular proteins, probes can mark selected molecular targets, and genetically encoded indicators can report cellular events such as calcium changes. Matching the labeling strategy to the research question allows investigators to connect molecular localization, neuronal structure, and activity-related signals within the same scientific framework.
Wavelength separation allows the microscope to distinguish fluorescence emitted by the label from the light used to excite it. Optical filters and related components isolate the emission signal, preserving contrast in the resulting image. This principle is especially important when examining fine neuronal features or localizing proteins, because unwanted illumination could otherwise obscure the labeled structures.
A basic workflow requires a fluorescent label suited to the target, an excitation-light source, optical elements that direct and collect light, and filters that separate emission from illumination. Depending on the experiment, researchers may prepare samples with antibodies, molecular probes, or genetically encoded indicators. The resulting images are then interpreted in relation to neuronal structure, protein location, or cellular activity.
Researchers can use labeled samples to visualize the shape and organization of neurons while identifying where selected proteins occur within those cells. Combining structural information with molecular localization helps relate changes in neuronal form to changes in protein distribution. This approach supports investigations of how cellular organization contributes to neuronal function and how it may change during disease-related processes.
Genetically encoded indicators can make changes in calcium concentration visible during observation. Because calcium-related signals provide information about cellular events, researchers can compare these signals with neuronal organization or other molecular features. Such measurements help connect dynamic changes inside neurons with their function, providing a bridge between fluorescence images and investigations of neural activity or disease mechanisms.