Infraorbital Nerve Ligation
Infraorbital Nerve Ligation - Related Videos
02:02
Inducing Orofacial Neuropathic Pain in a Rat Model via Infraorbital Nerve Ligations
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2025
This video demonstrates the induction of orofacial neuropathic pain in a rat model by ligating the infraorbital nerve. This results in nerve damage and inflammation, causing abnormal pain signaling and mimicking chronic pain conditions, which can be used to study the orofacial pain assay.
04:39
Induction of Neuropathic Pain in a Rat Model by Constriction of the Infraorbital Nerve
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2025
Source: Deseure, K., et al. Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain. J. Vis. Exp. (2015).This video demonstrates a method to induce neuropathic pain in a rat model by constricting the infraorbital nerve. After exposing the infraorbital nerve and tying ligatures to constrict it, the immune response at the injury site increases the excitability of neighboring neurons, which transmit pain signals to the brain.
10:52
Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain
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Cited by 45 •
2015
This manuscript describes a rodent model to study trigeminal neuropathic pain. The methods described include surgical procedures to perform a chronic constriction injury of the rat’s infraorbital nerve and the post-surgical behavioral tests to measure the changes in spontaneous and evoked behavior that are indicative of persistent pain and allodynia.
07:34
DNA Ligation Reactions
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2023
In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as a ligase catalyzes the ligation reaction. In the cell, ligases repair single and double strand breaks that occur during DNA replication. In the laboratory, DNA ligase is used during molecular cloning to join DNA fragments of inserts with vectors – carrier DNA molecules that will replicate target fragments in host organisms. This video provides an...
05:38
Chronic Constriction Injury of the Distal Infraorbital Nerve (DIoN-CCI) in Mice to Study Trigeminal Neuropathic Pain
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Cited by 7 •
2024
Chronic constriction injury of the distal infraorbital nerve in mice induces changes in spontaneous behavior (increased face grooming activity) and nocifensive behavior in response to tactile stimulation (hyperresponsiveness to von Frey hair stimulation) that are signs of ongoing pain and allodynia and serves as a model for trigeminal neuropathic pain.
FAQs
Partial constriction disrupts normal axonal signaling without fully severing the infraorbital nerve. This creates a localized injury state in which sensory information from the facial region can be processed abnormally. The resulting imbalance helps researchers examine how peripheral nerve damage initiates persistent changes in trigeminal sensory transmission rather than studying complete nerve transection.
The model can produce facial mechanical allodynia, meaning normally nonpainful mechanical stimulation becomes painful, along with spontaneous pain-like behaviors. These outcomes provide complementary indicators of altered nociception: evoked responses reveal sensitivity to external stimulation, whereas spontaneous behaviors suggest ongoing discomfort. Together, they help characterize behavioral consequences of trigeminal nerve injury.
The infraorbital nerve belongs to the maxillary division of the trigeminal nerve, so its injury provides a focused way to examine sensory processing within the trigeminal system. Researchers can therefore connect local peripheral damage with broader changes in trigeminal signaling, including mechanisms relevant to facial pain and altered sensory responses.
The procedure requires surgical exposure of the infraorbital nerve followed by placement of a ligature that partially constricts it. The ligature is intended to create injury while avoiding complete severing of the nerve. This sequence establishes a localized peripheral lesion suitable for subsequent assessment of facial pain-like behavior and sensory changes.
Researchers use this approach when they need an experimental model of trigeminal neuropathic pain caused by peripheral nerve injury. It supports investigation of nerve-injury mechanisms, neuroimmune interactions, and candidate treatments. Its relevance extends to post-traumatic trigeminal neuropathy and other orofacial pain disorders in which altered facial sensory processing is important.
Studies can assess whether nerve injury is associated with facial mechanical allodynia, spontaneous pain-like behaviors, and altered processing within the trigeminal pathway. These findings help link a localized peripheral lesion to measurable behavioral and neural consequences. The model can also provide a framework for evaluating potential treatments aimed at injury-related orofacial pain.