Orthotopic Allograft
Orthotopic Allograft - Related Videos
05:10
Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
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Cited by 5 •
2010
This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.
06:20
Syngeneic Mouse Orthotopic Allografts to Model Pancreatic Cancer
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Cited by 4 •
2022
Syngeneic mouse orthotopic allografts of pancreatic ductal adenocarcinoma (PDAC) recapitulate the biology, phenotypes, and therapeutic responses of disease subtypes. Owing to their fast, reproducible tumor progression, they are widely used in preclinical studies. Here, we show common practices to generate these models, injecting syngeneic murine PDAC cultures into the pancreas.
06:59
A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection
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Cited by 2 •
2022
The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.
07:15
Orthotopic Hind Limb Transplantation in the Mouse
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Cited by 24 •
2016
This novel model for orthotopic hind limb transplantation in the mouse, applying a non-suture cuff technique for super-microvascular anastomosis, provides a powerful tool for in vivo mechanistic immunological research related to vascularized composite allotransplantation (VCA).
10:01
Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
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Cited by 20 •
2012
Obliterative bronchiolitis is the key impediment to the long-term survival of lung transplant recipients and the lack of a robust preclinical model precludes examining obliterative bronchiolitis immunopathogenesis. Unlike other solid organ transplants, vascularized mouse lung transplantation has only recently been developed. Here we show our independently developed obliterative bronchiolitis model after murine orthotopic single-lung transplantation.
FAQs
The recipient’s local tissue environment strongly affects whether donor neural tissue or cells survive and integrate. Relevant conditions include the state of the surrounding neural tissue, available connectivity, and the recipient’s immune response. Because the graft is placed in a matching anatomical region, researchers can examine how that regional environment supports or limits circuit repair and graft-host interactions.
Placement in the corresponding brain or spinal cord region allows the graft to interact with the local neural circuitry rather than an unrelated environment. This makes it possible to study whether transplanted cells establish connections appropriate to that region and whether those connections contribute to functional recovery. Anatomical matching therefore links graft behavior to circuit-level repair.
The recipient’s immune response is a major factor in determining graft survival and integration. Since the donor and recipient are genetically different, immune-related graft-host interactions must be considered when interpreting neural transplantation results. Studying this response helps researchers distinguish effects caused by the transplanted cells from effects associated with the recipient’s reaction to the graft.
Post-transplant evaluation focuses on whether the donor tissue or cells remain viable, integrate with the host, and establish neural connections. Researchers can also examine graft-host interactions and evidence of functional recovery. Together, these outcomes indicate whether the transplanted material is merely present or is participating in circuit repair within the recipient’s brain or spinal cord.
These models provide a way to investigate how transplanted neural tissue or cells behave within a disease-relevant region of the nervous system. Researchers can use them to examine graft survival, circuit interactions, and potential repair mechanisms in the context of neurological disease. The resulting observations help clarify how transplantation might influence damaged neural systems.
Orthotopic allograft models connect cellular transplantation with anatomical circuit repair, making them useful for testing regenerative strategies before broader application. They allow researchers to assess survival, integration, graft-host interactions, and functional recovery in the nervous system. This combination of cellular and circuit-level information supports preclinical investigation of approaches intended to repair neurological damage.