A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Division of Translational Cancer Research,German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), 2Translational Cancer Research and Institute of Experimental Cancer Therapy, Klinikum rechts der Isar, School of Medicine,Technical University of Munich, 3Center for Translational Cancer Research (TranslaTUM), School of Medicine,Technical University of Munich, 4Department of Internal Medicine II, Klinikum rechts der Isar,Technical University of Munich
Chapters
- 00:04Introduction
- 00:46Preparing the Cell Lines Before Implantation
- 01:57Orthotopic Implantation of PDAC Cells
- 04:53Results: Syngeneic Mouse Orthotopic Allografts
- 05:39Conclusion
Syngeneic mouse orthotopic allografts of pancreatic ductal adenocarcinoma (PDAC) recapitulate the biology, phenotypes, and therapeutic responses of disease subtypes. Owing to their fast, reproducible tumor progression, they are widely used in preclinical studies. Here, we show common practices to generate these models, injecting syngeneic murine PDAC cultures into the pancreas.
