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Syngeneic Mouse Orthotopic Allografts to Model Pancreatic Cancer
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Syngeneic Mouse Orthotopic Allografts to Model Pancreatic Cancer
DOI:

06:20 min

October 04, 2022

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Chapters

  • 00:04Introduction
  • 00:46Preparing the Cell Lines Before Implantation
  • 01:57Orthotopic Implantation of PDAC Cells
  • 04:53Results: Syngeneic Mouse Orthotopic Allografts
  • 05:39Conclusion

Summary

Automatic Translation

Syngeneic mouse orthotopic allografts of pancreatic ductal adenocarcinoma (PDAC) recapitulate the biology, phenotypes, and therapeutic responses of disease subtypes. Owing to their fast, reproducible tumor progression, they are widely used in preclinical studies. Here, we show common practices to generate these models, injecting syngeneic murine PDAC cultures into the pancreas.

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