Rotor Preparation
Rotor Preparation - Related Videos
09:45
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Cited by 17 •
2012
Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.
09:26
How to Build a Vacuum Spring-transport Package for Spinning Rotor Gauges
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Cited by 1 •
2016
Here we describe how to build a robust spring-transport mechanism for a spinning rotor gauge. This device securely immobilizes the rotor and keeps it under vacuum during transportation. We also describe packaging that minimizes the risk of damage during transport. Tests show our design works for typical shocks during transport.
07:45
Preparation and Fractionation of Xenopus laevis Egg Extracts
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Cited by 7 •
2008
Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
06:23
A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice
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Cited by 8 •
2020
Presented here is a protocol for chronic sleep fragmentation (CSF) model achieved by an electrically controlled orbital rotor, which could induce confirmed cognitive deficit and anxiety-like behavior in young wild-type mice. This model can be applied to explore the pathogenesis of chronic sleep disturbance and related disorders.
08:30
Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
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Cited by 43 •
2011
A method to prepare translationally active, intact synaptoneurosomes (SNs) from mouse brain cortex is described. The method uses a discontinuous Percoll-sucrose density gradient allowing for the quick preparation of active SNs.
FAQs
Inspection identifies damage that could compromise safe operation or reduce the reproducibility of separation. This check addresses the physical condition of the rotor and buckets, while sample balancing addresses mass distribution. Performing both checks before use helps reduce the risk of equipment damage and sample loss during the intended centrifugation step.
Balancing by mass helps maintain safe operation and consistent separation conditions across the rotor. Samples that differ in mass can make the centrifugation step less reliable, even when the tubes appear similar in volume. Careful mass matching therefore supports reproducible recovery of brain-tissue or cultured-neuron fractions for later analysis.
Tube selection depends on compatibility with the rotor, while the centrifugation conditions must match the intended separation step. These choices should be made together rather than independently, because unsuitable tubes or mismatched settings can undermine sample recovery and reproducibility. Confirming compatibility and conditions is especially important when processing distinct neural subcellular fractions.
A practical sequence begins by checking the rotor and buckets for damage, followed by selecting compatible tubes and balancing the samples by mass. The operator then secures the lid and sets conditions appropriate for the planned centrifugation step. Completing these actions before starting the run creates a consistent preparation process and helps prevent avoidable sample loss or equipment damage.
In neuroscience workflows, prepared rotors support separations involving nuclei, synaptosomes, membrane fractions, proteins, and other subcellular components. These materials may originate from brain tissue or cultured neurons. Careful handling is relevant whenever the separated material will be used in biochemical, imaging, or molecular analyses, where inconsistent recovery could affect downstream interpretation.
Consistent preparation improves the reliability of material recovered from a centrifugation step and reduces preventable sample loss. That reliability matters when isolated fractions are carried forward into biochemical assays, imaging, or molecular analyses. By combining condition matching, tube compatibility, mass balancing, and secure closure, the workflow supports more dependable comparisons between samples.