Transgenic Mouse Model
Transgenic Mouse Model - Related Videos
04:03
Triggering and Monitoring Heat-Induced Seizures in a Transgenic Mouse Model
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2025
This video demonstrates the procedure to induce heat-induced seizures in a transgenic mouse with epilepsy-related gene mutations by gradually increasing body temperature in a controlled chamber. This protocol is used to study seizure mechanisms and potential therapies for epilepsy.
03:07
Bioluminescence Imaging of Neuroinflammation in a Transgenic Mouse
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2025
Source: Breid, S., et. al. Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils. J. Vis. Exp. (2017)This video demonstrates the bioluminescence imaging of neuroinflammation in transgenic mice, showcasing luciferase-luciferin reactions in activated astrocytes. The protocol enables non-invasive quantification of astrocytic activation, aiding studies on CNS inflammation and therapeutic efficacy.
08:16
An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses
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Cited by 14 •
2017
The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct.
13:59
Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
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Cited by 76 •
2010
The intensely studied nematode worm Caenorhabditis elegans can be transgenically engineered to express the human β-amyloid peptide (Aβ). Induced expression of Aβ in C. elegans muscle leads to a rapid, reproducible paralysis phenotype that can be used to monitor treatments that modulate Aβ toxicity.
08:51
Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
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Cited by 14 •
2012
Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.
FAQs
Tissue-specific promoters can restrict expression to selected cell types, giving investigators a way to examine gene effects in defined neuronal populations. This focus is important when the scientific question concerns neuronal development or synaptic signaling, because observations can be related to the cells in which the engineered gene is active. It therefore supports more targeted interpretation of neural changes.
By comparing neuronal development, synaptic signaling, and behavior in mice carrying different gene changes, investigators can examine how a particular gene contributes to brain function. The living-animal setting connects the genetic alteration with outcomes across several levels of neuroscience. This makes the model useful for moving from a gene-centered question toward an integrated account of neural function or dysfunction.
Once successfully modified mice transmit the engineered change to offspring, researchers can study the same genetic condition across related animals and maintain the model for experiments involving brain development, signaling, behavior, or disease. This inherited continuity is especially useful when asking whether observed findings are associated with the engineered genetic change rather than with a single generation.
The choice to introduce, remove, or alter a gene changes the biological question the model can address. A researcher may investigate what happens when a gene is present in a modified form, absent, or changed. In neuroscience, that distinction helps organize studies of gene function in development, synaptic signaling, behavior, and neurological disease.
A typical workflow starts by introducing genetic material into embryonic cells or early embryos. Researchers then identify mice in which the intended modification was successfully established and determine whether those animals can transmit it to offspring. This process produces animals suitable for controlled studies of the engineered gene, including its relationship to neural development, signaling, behavior, or disease.
These models are useful when a neuroscience study needs to connect a gene with a specific brain-related question. Applications include examining neuronal development, synaptic signaling, and behavior, as well as investigating neurological disease mechanisms. They can also support evaluation of therapeutic targets and analysis of how genetic factors contribute to brain disorders under controlled experimental conditions.
Observed changes in neuronal development, synaptic signaling, behavior, or disease-related processes can be interpreted in relation to the engineered genetic change. Because experiments occur under controlled conditions, researchers can use the model to examine disease mechanisms and assess therapeutic targets while considering genetic contributions to brain disorders. Its value lies in linking the manipulation to neuroscience outcomes.