Hippocampal Volume
Hippocampal Volume - Related Videos
09:51
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
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Cited by 5 •
2018
This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal slices.
08:20
Organotypic Hippocampal Slice Cultures
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Cited by 52 •
2011
We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.
17:46
Low-Density Primary Hippocampal Neuron Culture
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Cited by 15 •
2017
This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.
05:13
Co-culturing of Ventral Hippocampal and Nucleus Accumbens
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2025
This video demonstrates the process of preparing ventral hippocampal and nucleus accumbens tissues from mouse brains for co-culture. Neurons extend projections to form synaptic connections between the ventral hippocampal and nucleus accumbens tissues. This method allows studying neuronal interactions between these two brain regions, offering insights into cognition, behavior, and neurological disorders.
10:27
Isolation and Culture of Hippocampal Neurons from Prenatal Mice
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Cited by 183 •
2012
We provide a protocol for the culture of highly purified hippocampal neurons from prenatal mouse brains without the use of a feeder glial cell layer.
FAQs
Overall brain size can differ among individuals, so an unadjusted measurement may reflect general anatomical differences rather than variation specific to the hippocampus. Adjusting hippocampal volume for brain size helps researchers make more meaningful comparisons across participants and better examine how hippocampal anatomy relates to memory, stress, aging, mood, trauma, or disease.
Differences in hippocampal volume provide a structural measure that psychologists can examine alongside memory formation and spatial learning. The measurement does not replace cognitive assessment; instead, it helps researchers investigate whether variation in hippocampal tissue is associated with differences in these abilities or with experiences and health conditions that influence cognitive function.
Repeated measurements allow researchers to track changes in hippocampal structure over time rather than relying on a single assessment. This approach can help examine patterns of brain change associated with aging, stress, mood disorders, trauma, or neurodegenerative disease, and can support evaluation of whether a potential treatment is accompanied by measurable structural change.
Volume estimates depend on identifying the hippocampal boundaries and calculating the tissue enclosed within them. Careful delineation is therefore central to producing a meaningful measurement: the resulting value represents the selected hippocampal region rather than an undifferentiated portion of the medial temporal lobe. This supports consistent comparisons in psychological and clinical research.
Researchers use structural magnetic resonance imaging to obtain images of brain anatomy, delineate the boundaries of the hippocampus, and calculate the amount of tissue enclosed by those boundaries. They may then adjust the measurement for overall brain size. The resulting estimate can be compared across people or across time in longitudinal studies.
Psychologists examine hippocampal volume when investigating how brain structure relates to stress, mood disorders, and trauma. They may also consider memory and cognitive function within these investigations. This structural measure helps connect psychological experiences or conditions with anatomical variation, while longitudinal designs can show how the hippocampus changes across the course of study.
Measurements of hippocampal volume can help researchers examine structural differences associated with aging and neurodegenerative disease. Repeated assessments add information about the direction and timing of brain change, while comparisons with cognitive measures can clarify how anatomical variation relates to memory and other aspects of cognitive function. These data also provide a basis for evaluating potential treatment effects.
A treatment study can use hippocampal volume as a structural outcome measured before and after an intervention, particularly when researchers want to track brain change over time. The measurement does not stand alone: relating volume changes to memory, psychological conditions, or other cognitive outcomes helps investigators assess whether treatment-related structural differences have functional relevance.