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Low-Density Primary Hippocampal Neuron Culture

Low-Density Primary Hippocampal Neuron Culture

Article DOI: 10.3791/55000
April 18th, 2017


Summary April 18th, 2017

This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.


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