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Chapters
- 00:00Title
- 01:19Glia Culture Preparation
- 05:57Preparing Coverslips for Neuron Culture
- 09:22Neuronal Culture Preparation
- 16:13Immunofluorescence Results for Neuronal Markers
- 17:01Conclusion
This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.
