条形码酵母文库的竞争性基因组筛选
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被引用 44 次
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11:59 分钟
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2011年8月11日
DOI :10.3791/2864-v
2011年8月11日
•19.6K 次观看
Andrew M. Smith*1,2 , Tanja Durbic*2,3 , Julia Oh*4 , Malene Urbanus1,2 , Michael Proctor5 , Lawrence E. Heisler2,3 , Guri Giaever2,6 , Corey Nislow1,2,3
1Banting and Best Department of Medical Research and Department of Molecular Genetics, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Donnelly Sequencing Centre, University of Toronto, 4Genetics and Molecular Biology Branch, National Human Genome Research Institute, NIH, 5Stanford Genome Technology Center, Stanford School of Medicine, Stanford University , 6Department of Pharmaceutical Sciences, University of Toronto
* These authors contributed equally
我们已开发出全面且无偏倚的全基因组筛选方法，以研究基因-药物和基因-环境之间的相互作用。本文介绍了对这些突变体文库进行筛选的方法。
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Chapters in this video
0:05
Title
1:55
Combining Individual Mutants into a Single Pool
3:23
Experimental Pool Growth
4:56
Genomic DNA Extraction, PCR and Microarray Hybridization
8:33
Sequencing Barcoded Yeast Strains
9:22
Genomic Screening Results
11:03
Conclusion
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