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Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

Article DOI: 10.3791/3602-v • 07:16 min • November 26th, 2011
November 26th, 2011 •
1, 2
1Department of Chemistry, Tunghai University, 2Department of Biochemistry, University of California, Riverside

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MALDI-TOF mass spectrometry was successfully utilized to monitor the amide hydrogen/deuterium exchange in protein kinase Pak2 activation.

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Amide Hydrogen/deuterium Exchange MALDI-TOF Mass Spectrometry Protein-protein Interactions Protein Conformational Changes Protein Dynamics Protein-ligand Interactions Deuteration Rates Pepsin Digestion Resolution Heteronuclear Single Quantum Coherence (HSQC) NMR Aqueous Solution MALDI-TOF Detection HPLC/ESI-MS System Protein Kinase Pak2 Gamma-Pak
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