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Generation of Mice Derived from Induced Pluripotent Stem Cells
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Generation of Mice Derived from Induced Pluripotent Stem Cells

Article DOI: 10.3791/4003-v • 11:56 min • November 29th, 2012
November 29th, 2012 •
1, 1, 1, 2, 2, 2, 1
1Dorris Neuroscience Center & Department of Cell Biology, The Scripps Research Institute, 2Mouse Genetics Core Facility, The Scripps Research Institute

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Generating induced pluripotent stem cell (iPSC) lines produces lines of differing developmental potential even when they pass standard tests for pluripotency. Here we describe a protocol to produce mice derived entirely from iPSCs, which defines the iPSC lines as possessing full pluripotency1.

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Induced Pluripotent Stem Cells IPSCs Basic Research Regenerative Therapies Somatic Cells Germ Layers Embryoid Bodies Teratomas Gene Expression Chimeric Mice Pluripotency Markers Donor Cell Sources Selection Methods Tetraploid Embryo Complementation Full Pluripotency Electrofusion Blastocyst Stage
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