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从人骨骼肌隔离血管源性多能前体

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

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生物学

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JoVE 杂志生物学

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

从人骨骼肌隔离血管源性多能前体

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11,580 Views

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10:52 min

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August 21, 2014

DOI:

10.3791/51195-v

DOI:

10.3791/51195-v

•

10:52 min

August 21, 2014

•

3 Views

William C.W. Chen¹, Arman Saparov^2,3, Mirko Corselli⁴, Mihaela Crisan⁵, Bo Zheng⁶, Bruno Péault^7,8, Johnny Huard⁹

¹Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery,University of Pittsburgh, ²Department of Orthopedic Surgery,University of Pittsburgh, ³Nazarbayev University Research and Innovation System,Nazarbayev University, ⁴Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center,University of California at Los Angeles, ⁵Department of Cell Biology,Erasmus MC Stem Cell Institute, ⁶OHSU Center for Regenerative Medicine,Oregon Health & Science University, ⁷Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine,Queen’s Medical Research Institute and University of Edinburgh, ⁸David Geffen School of Medicine and the Orthopaedic Hospital Research Center,University of California at Los Angeles, ⁹Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine,University of Pittsburgh

Summary

人体骨骼肌肉的海港几个多系前驱体的人口，是理想的可再生应用血管内。这种隔离方法允许3多能前体细胞群的同时纯化分别从血管三个结构层次:从内膜肌内皮细胞，从媒体的周细胞，并从外膜外膜细胞。

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