FIBS-enabled Noninvasive Metabolic Profiling

Alireza Behjousiar; Antony Constantinou; Karen M. Polizzi; Cleo Kontoravdi

doi:10.3791/51200

JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering

FIBS-enabled Noninvasive Metabolic Profiling

FIBS功能的无创性代谢轮廓

Full Text

10,316 Views

09:16 min

February 3, 2014

DOI: 10.3791/51200-v

Alireza Behjousiar^1,2, Antony Constantinou², Karen M. Polizzi², Cleo Kontoravdi¹

¹Centre for Process Systems Engineering, Department of Chemical Engineering and Chemical Technology,Imperial College London, ²Centre for Synthetic Biology and Innovation, Division of Molecular Biosciences,Imperial College London

Please note that some of the translations on this page are AI generated. Click here for the English version.

如何在原位代谢轮廓校正共振能量转移集成生物传感器（FIBS）用于描述呈现。该FIBS可用于测量非侵入性代谢物辅助的代谢模式和生物过程条件的高通量筛选的发展的细胞内水平。

该程序的总体目标是使用基于基因编码的蛋白质生物传感器无创监测细胞内葡萄糖或谷氨酰胺的水平。这是通过首先用含有质粒的生物传感器基因横切目标细胞并选择表达生物传感器的细胞来实现的。在第二步中，从分批或补料分批培养物中提取细胞培养样品，并测量其品丝比。

然后可以通过独立测定法测定样品的细胞内葡萄糖或谷氨酰胺浓度。最终，样品的品丝比可用于无创实时计算葡萄糖或谷氨酰胺的细胞内浓度。该技术的意义延伸到生物过程的改进，因为这是获取可用于在线优化和控制的实时代谢信息的可靠方法。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

生物工程 84期代谢物的监测在体内生物传感器原位监控哺乳动物细胞培养生物过程工程培养基配方