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Afleiding van Cardiac stamcellen uit embryonale stamcellen
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells
DOI:

08:00 min

January 12, 2015

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Chapters

  • 00:05Title
  • 01:16Induction of Mouse Cardial Progenitor Cell (OPC) Differentiation by Embryoid Body (EB) Formation
  • 04:47Induction of Human CPC Differentiation by EB Formation
  • 06:28Results: Differentiation of ESCs into Multipotent CPCs
  • 07:27Conclusion

Summary

自动翻译

In this protocol, derivation of cardiac progenitor cells from both mouse and human embryonic stem cells will be illustrated. A major strategy in this protocol is to enrich cardiac progenitor cells with flow cytometry using fluorescent reporters engineered into the embryonic stem cell lines.

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