Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice

Maria E. Laucho-Contreras; Katherine L. Taylor; Ravi Mahadeva; Steve S. Boukedes; Caroline A. Owen

doi:10.3791/52236

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Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice

肺气肿，小气道重塑的香烟烟雾暴露小鼠自动测量

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10:37 min

January 16, 2015

DOI: 10.3791/52236-v

Maria E. Laucho-Contreras¹, Katherine L. Taylor¹, Ravi Mahadeva², Steve S. Boukedes³, Caroline A. Owen^1,4

¹Division of Pulmonary and Critical Care Medicine,Brigham and Women's Hospital - Harvard Medical School, ²Department of Respiratory Medicine,University of Cambridge - Addenbrooke's Hospital, ³Lung Transplant Program,Brigham and Women's Hospital - Harvard Medical School, ⁴COPD and IPF Programs,Lovelace Respiratory Research Institute

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该协议的目标是提供自动化方法来量化 COPD 小鼠模型中的慢性肺部病变。该方案包括将小鼠暴露于香烟烟雾（CS）、测量肺功能、给肺充气，以及使用形态测量方法测量小鼠的肺气肿和小气道重塑。

该程序的目的是量化 COPD，就像暴露于香烟烟雾的小鼠肺部的慢性肺部病变一样。这是通过首先将小鼠暴露在香烟烟雾或空气中六个月来实现的。接下来，使用啮齿动物呼吸机对小鼠进行肺功能测试。

然后将肺充气至固定压力，去除并固定在 10% 福尔马林中。最后，使用更多信息软件来测量鳃染色肺切片和小气道细胞外基质、蛋白质层厚度或梅森毛状体染色肺切片上的气腔大小。最终，以标准化、无偏倚和自动化的方式测量暴露于香烟烟雾的小鼠肺部气腔扩大。

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