A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

Elisa Gecchele; Matilde Merlin; Annalisa Brozzetti; Alberto Falorni; Mario Pezzotti; Linda Avesani

doi:10.3791/52459

Journal

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生物学

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细菌，昆虫细胞和植物系统:重组蛋白表达在不同的生物工厂的比较分析

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

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生物学

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JoVE 杂志生物学

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

细菌，昆虫细胞和植物系统:重组蛋白表达在不同的生物工厂的比较分析

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23,505 Views

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09:11 min

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March 23, 2015

DOI:

10.3791/52459-v

DOI:

10.3791/52459-v

•

09:11 min

March 23, 2015

•

23468 Views

Elisa Gecchele*¹, Matilde Merlin*¹, Annalisa Brozzetti², Alberto Falorni², Mario Pezzotti¹, Linda Avesani¹

¹Department of Biotechnology,University of Verona, Verona, Italy ²Department of Internal Medicine,University of Perugia, Perugia, Italy

Summary

In this study the expression of a target human recombinant protein in different production platforms was compared. We focused on traditional fermenter-based cultures and on plants, describing the set-up of each system and highlighting, on the basis of the reported results, the inherent limits and advantages for each platform.