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Immunology and Infection

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Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Article DOI: 10.3791/52607-v • 11:44 min • March 24th, 2015
March 24th, 2015 •
*1, *1, 1, 1, 1, 1, 1, 2, 3, 1, 4, 1
1Department of Medicine III, RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging", RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery, RWTH Aachen University, 4Institute for Pharmacology, RWTH University-Hospital Aachen
* These authors contributed equally

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Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

Tags

Intravital Microscopy Multiphoton Microscopy Immune Cells Liver Inflammation Leukocytes Monocytes Neutrophils T Cells B Cells Natural Killer Cells NKT Cells Immune Cell Migration Inflammatory Liver Disease Cell-cell Interactions Two-photon Laser Scanning Microscopy (TPLSM) Intensive Care Monitoring Photobleaching Phototoxic Effects
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