Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Siriphan Manocheewa; Erinn C. Lanxon-Cookson; Yi Liu; J. Victor Swain; Jan McClure; Ushnal Rao; Brandon Maust; Wenjie Deng; Justine E. Sunshine; Moon Kim; Morgane Rolland; James I. Mullins

doi:10.3791/52610

Journal

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免疫与感染

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成对增长竞争测定法测定人类免疫缺陷病毒的复制健身

JoVE 杂志
免疫与感染

This content is Free Access.

JoVE 杂志免疫与感染

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

成对增长竞争测定法测定人类免疫缺陷病毒的复制健身

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11,065 Views

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11:19 min

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May 04, 2015

DOI:

10.3791/52610-v

DOI:

10.3791/52610-v

•

11:19 min

May 04, 2015

•

3 Views

Siriphan Manocheewa¹, Erinn C. Lanxon-Cookson¹, Yi Liu¹, J. Victor Swain¹, Jan McClure¹, Ushnal Rao¹, Brandon Maust¹, Wenjie Deng¹, Justine E. Sunshine¹, Moon Kim¹, Morgane Rolland^3,4, James I. Mullins^1,2

¹Department of Microbiology,University of Washington, ²Departments of Medicine and Laboratory Medicine,University of Washington, ³U.S Military HIV Research Program,Walter Reed Army Institute of Research, ⁴Henry M. Jackson Foundation

Summary

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.