Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Thomas S. Lisse; Elizabeth A. Brochu; Sandra Rieger

doi:10.3791/52654

Journal

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发育生物学

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捕获组织修复的斑马鱼幼虫与时间推移明视立体显微镜

JoVE 杂志
发育生物学
Author Produced

This content is Free Access.

JoVE 杂志发育生物学

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

捕获组织修复的斑马鱼幼虫与时间推移明视立体显微镜

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10,608 Views

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14:29 min

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January 31, 2015

DOI:

10.3791/52654-v

DOI:

10.3791/52654-v

•

14:29 min

January 31, 2015

•

3 Views

Thomas S. Lisse¹, Elizabeth A. Brochu¹, Sandra Rieger¹

¹Davis Center for Regenerative Biology and Medicine,MDI Biological Laboratory

Summary

We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.