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Custom-made Microdialysis Probe Design
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神经科学
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JoVE 杂志 神经科学
Custom-made Microdialysis Probe Design

Custom-made Microdialysis Probe Design

DOI:
10.3791/53048-v

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05:38 min

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July 21, 2015

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, , , ,
1Department of Pharmacology,Goethe University of Frankfurt, 2Department of Pharmaceutical Chemistry,Goethe University of Frankfurt, 3Department of Clinical Pharmacology,Goethe University of Frankfurt

Chapters

  • 00:05Title
  • 01:18Preparaing PTFE-tubing
  • 01:50Preparation and Placement of Fused Silica
  • 02:37Applying the Membrane
  • 03:46Applying the Metal Sleeve
  • 04:23Results: In Vitro Probe Recovery of Small Molecules
  • 05:13Conclusion

Summary

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Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.

Tags

Microdialysis ProbeCustom-madePolysulfone MembraneMolecular WeightIn Vitro RecoveryNeuroscience ResearchCerebrospinal FluidNeuropeptidesGlucoseLactateAcetylcholineATPMolecular Cut-off

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