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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Article DOI: 10.3791/55157-v • 08:06 min • January 19th, 2017
January 19th, 2017 •
1, 1, 2, 1
1Dept. of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem, 2The Core Research Facility, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem

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We describe here a relatively fast and simple approach for mapping genome-wide mammalian replication timing, from cell isolation to the basic analysis of the sequencing results. A genomic map of a representative replication program will be provided following the protocol.

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Genome-wide Replication Timing DNA Content Measurement Health And Disease Fast And Simple Cells Procedure Dan Lehmann Faculty Of Medicine Adherent Cells PBS Trypsin-EDTA Centrifuge Cold PBS
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