Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

Jing Gao; Xiaolu Shi; Hong He; Juhong Zhang; Ding Lin; Guosheng Fu; Dongwu Lai

doi:10.3791/55797

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Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

肌网钙储备与细胞内舒张钙在心室肌细胞中的分离评价

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11:00 min

September 18, 2017

DOI: 10.3791/55797-v

Jing Gao*¹, Xiaolu Shi*², Hong He¹, Juhong Zhang¹, Ding Lin³, Guosheng Fu¹, Dongwu Lai¹

¹Department of Cardiology, Sir Run Run Shaw Hospital,Zhejiang University School of Medicine, ²Experimental Research Center,China Academy of Chinese Medical Sciences, ³Department of Cardiology,The Third Hospital of Hangzhou City

Please note that some of the translations on this page are AI generated. Click here for the English version.

细胞内钙循环对心肌细胞收缩和舒张功能的调节起着至关重要的作用。在这里, 我们描述了一个协议, 以评估肌网钙^{2 +}储备和舒张钙功能的心肌细胞钙显像系统。

该程序的总体目标是评估离体心肌细胞的肌浆网钙储备和细胞内舒张期钙去除功能。该方法可以帮助回答在某些病理生理条件（如糖尿病和心力衰竭）下细胞内钙处理机制的关键问题。这种技术的主要优点是方便，受技术和设备的限制较小。

要开始此过程，请设置 Langendorff 灌注系统。用 NT 溶液填充灌注系统，将温度设置为 36.5 摄氏度，并消除管内的任何气泡。接下来，在剑突下打开腹腔，提起剑突，用剪刀打开胸部。

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细胞生物学问题 127 肌网钙稳态钙储备细胞内钙去除 SERCA 心肌细胞