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JoVE Journal Biochemistry
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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Article DOI: 10.3791/59152-v • 09:37 min • February 12th, 2019
February 12th, 2019 •
*1, *1, 2, 3, 1, 1
1Department of Chemistry, Louisiana State University, 2National High Magnetic Field Laboratory, 3Departments of Pediatrics, and Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center
* These authors contributed equally

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A protocol for preparing 13C,15N-labeled fungal and plant samples for multidimensional solid-state NMR spectroscopy and dynamic nuclear polarization (DNP) investigations is presented.

Tags

Structural Elucidation Fungal Materials Plant Materials Solid-state NMR Dynamic Nuclear Polarization Biosystem Characterization Atomic Resolution Antifungal Drugs Complex Carbohydrates Fungal Contamination NMR Cell Culture Liquid Growth Medium Solid Growth Medium
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