Journal
/
/
牵引显微镜与微流体集成,用于化学集体迁移
JoVE 杂志
生物工程
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物工程
Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
DOI:

10:53 min

October 13, 2019

, , , , ,

Chapters

  • 00:04Title
  • 00:47Fabrication of Polydimethylsiloxane (PDMS) Stencil and Microchannel
  • 02:39Preparation of Bottom Glass with Polyacrylamide (PA) Gel
  • 04:41Micropatterning of Cell Islands and Assembly of PA Gel with PDMS Microchannel
  • 06:14Integrated Microfluidic System
  • 07:32Image Acquisition
  • 08:27Results: Mechanical Interactions of MDCK Cell Islands
  • 09:55Conclusion

Summary

自动翻译

发育、伤口愈合和癌症转移中的集体细胞迁移通常受生长因子或信号分子梯度的引导。本文介绍了一个实验系统,将牵引显微镜与微流体系统相结合,并演示了如何在生物化学梯度下量化集体迁移的力学。

Related Videos

Read Article