Journal
/
化学
/
Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
JoVE 杂志
化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 化学
Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

DOI:
10.3791/62672-v

•

05:45 min

•

June 08, 2021

•

, , , , ,
1School of Chemistry and Materials Science,Nanjing Normal University, 2Institute for Cell Analysis,Shenzhen Bay Laboratory, 3Department of Chemistry,University of British Columbia

Chapters

  • 00:04Introduction
  • 00:38Operation of CE-Based HDX MS Analysis
  • 04:01Results: Conformational Characterization of Proteins with Top-Down Mass Spectrometry
  • 05:18Conclusion

Summary

自动翻译

自动翻译

Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation.

Tags

Capillary ElectrophoresisHydrogen/deuterium ExchangeConformational CharacterizationTop-down Mass SpectrometryProtein Species SeparationHigher-order StructureHDX-CE-MSElectrophoretic EffectPreconditioningUltrasonicationBackground ElectrolyteInfusion TubingNano-electrospray IonizationInjection Volume EstimationElectrophoretic Voltage

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Read Article