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In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
DOI:

10:20 min

November 18, 2022

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Chapters

  • 00:05Introduction
  • 02:22Calibration of the OCTOPUS
  • 03:49Programming One Experimental Process in DINATEN for Each Digestive Step
  • 05:51Setting up the Experiment
  • 06:41Results: Gastric Digestion, Intestinal Digestion, and Complete Dynamic Gastrointestinal Digestion Profiles and Final Values of Gastrointestinal Digestion
  • 09:22Conclusion

Summary

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A pendant drop surface film balance implemented with a multi-subphase exchange, nicknamed the OCTOPUS, allows for mimicking digestive conditions by the sequential subphase exchange of the original bulk solution with simulated gastrointestinal fluids. The simulated in vitro digestion is monitored by recording in situ the interfacial tension of the digested interfacial layer.

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