Journal
/
Bioengineering
/
Gelatin Methacryloyl Granular Hydrogel Scaffolds: High-throughput Microgel Fabrication, Lyophilization, Chemical Assembly, and 3D Bioprinting
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Gelatin Methacryloyl Granular Hydrogel Scaffolds: High-throughput Microgel Fabrication, Lyophilization, Chemical Assembly, and 3D Bioprinting

Gelatin Methacryloyl Granular Hydrogel Scaffolds: High-throughput Microgel Fabrication, Lyophilization, Chemical Assembly, and 3D Bioprinting

DOI:
10.3791/64829-v

•

10:36 min

•

December 09, 2022

•

, , , ,
1Department of Chemical Engineering,The Pennsylvania State University, 2Department of Biomedical Engineering,The Pennsylvania State University

Chapters

  • 00:04Introduction
  • 00:58Droplet Formation and GelMA Microgel Fabrication
  • 02:45Converting the Microgels to Resuspendable Powder by the Microengineered Emulsion-to-Powder (MEtoP) Technology
  • 03:55GelMA Granular Hydrogel Scaffold (GHS) Formation
  • 04:55Nanoengineered Granular Bioink (NGB) with Preserved Microporosity
  • 08:33Results: Analysis of GelMA Microgels, GHS, and NGB
  • 10:04Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes protocols for high-throughput gelatin methacryloyl microgel fabrication using microfluidic devices, converting microgels to resuspendable powder (micro-aerogels), the chemical assembly of microgels to form granular hydrogel scaffolds, and developing granular hydrogel bioinks with preserved microporosity for 3D bioprinting.

Tags

Keywords: Gelatin MethacryloylGelMAHydrogel ScaffoldsMicrogel FabricationLyophilizationChemical Assembly3D BioprintingExtracellular MatrixECMPhotochemical CrosslinkingCell-responsiveBiodegradableMicroporousCell Infiltration3D BioprintingRegenerative EngineeringLAPPhotoinitiatorDPBSBiocompatible SurfactantPDMSSyringe PumpOptical MicroscopyPhysical CrosslinkingMEtoP

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/cn/64829?article_type=v

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
check_url/cn/64829?article_type=v

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
check_url/cn/64829?article_type=v

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization
check_url/cn/64829?article_type=v

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
check_url/cn/64829?article_type=v

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds
check_url/cn/64829?article_type=v

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
check_url/cn/64829?article_type=v

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering
check_url/cn/64829?article_type=v

Interlinked Macroporous 3D Scaffolds from Microgel Rods
check_url/cn/64829?article_type=v

Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
check_url/cn/64829?article_type=v

Synthesis of Strong Adhesive Hydrogel, Gelatin O-Nitrosobenzaldehyde

Read Article