颅内插管植入小鼠连续局部区域嵌合抗原受体（CAR）T细胞输注

Overview

This protocol outlines a method for locoregional cannula implantation in mice, facilitating the preclinical evaluation of immunotherapeutic infusions targeting central nervous system tumors. The technique allows for repeated doses of CAR T-cell therapy without the need for multiple invasive surgeries.

Key Study Components

Area of Science

• Neuroscience
• Oncology
• Immunotherapy

Background

• CNS tumors are the leading cause of cancer-related deaths in children.
• Locoregional immune-based therapies are being tested in clinical trials.
• CAR T-cell therapy shows promise in targeting CNS tumors.
• This protocol replicates an intraventricular catheter system used in ongoing clinical trials.

Purpose of Study

• To evaluate the effectiveness of CAR T-cell therapy in a preclinical model.
• To provide a method for local regional delivery of therapeutics.
• To minimize the invasiveness of repeated treatments in animal models.

Methods Used

• Implantation of a cannula using a stereotactic apparatus.
• Administration of CAR T-cell therapy through the implanted cannula.
• Use of anesthesia and surgical techniques to ensure animal welfare.
• Monitoring of tumor response and survival outcomes post-treatment.

Main Results

• The GPC2-directed CAR T-cell therapy induced significant tumor regression.
• Significantly prolonged survival was observed in the thalamic diffuse midline glioma model.
• The method allows for precise delivery of treatment to targeted areas.
• Insights gained may inform future research in neuroscience and oncology.

Conclusions

• This protocol provides a valuable tool for studying CNS tumors.
• It enhances the understanding of CAR T-cell therapy efficacy.
• The approach may lead to improved therapeutic strategies for CNS tumors.

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