High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

Melissa L. Blotter; Isaac W. Stubbs; Jacob H. Norby; Maxwell Holmes; Ben Kearsley; Alexis Given; Kutter Hine; Micah R. Shepherd; R. Ryley Parrish

doi:10.3791/67065

使用互补金属氧化物半导体高密度微电极阵列系统从急性脑切片中获得高质量的癫痫样活性

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

使用互补金属氧化物半导体高密度微电极阵列系统从急性脑切片中获得高质量的癫痫样活性

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06:28 min

September 27, 2024

DOI: 10.3791/67065-v

Melissa L. Blotter*^1,2, Isaac W. Stubbs*^1,2, Jacob H. Norby*^1,2, Maxwell Holmes^1,2, Ben Kearsley³, Alexis Given¹, Kutter Hine^1,4, Micah R. Shepherd⁵, R. Ryley Parrish^1,2

¹Department of Cell Biology and Physiology,Brigham Young University, ²Neuroscience Center,Brigham Young University, ³Department of Statistics,Brigham Young University, ⁴Department of Biology,Brigham Young University, ⁵Department of Physics and Astronomy,Brigham Young University

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Overview

This study outlines a protocol for using complementary metal-oxide-semiconductor high-density microelectrode array systems (CMOS-HD-MEAs) to investigate seizure-like activity from ex vivo brain slices. The research aims to enhance the understanding of seizure initiation, propagation, and termination, with a focus on developing novel therapies for status epilepticus.

Key Study Components

Area of Science

Neuroscience
Electrophysiology
Epileptology

Background

Seizures can have complex initiation and propagation mechanisms.
Status epilepticus is a critical condition that often resists treatment.
High-density microelectrode arrays provide detailed electrophysiological data.
Different paradigms offer insights into the dynamics of seizure-like activity.

Purpose of Study

To establish a reliable protocol for recording seizure activity.
To explore the spatial and temporal patterns of status epilepticus.
To inform the development of more effective treatments for severe seizures.

Methods Used

The main platform used is CMOS-HD-MEAs with ex vivo brain slices.
Brain slices are subjected to various treatment paradigms to investigate seizure-like activity.
Detailed preparation and maintenance steps are provided for optimal recordings.
The protocol includes steps for preparing the microelectrode array and ensuring proper environmental conditions.
Electrophysiological activity is recorded to analyze seizure characteristics.

Main Results

Neocortical regions show significant electrographic seizure-like activity under specific conditions.
Hippocampal regions exhibited variability in seizure-like activity.
Different paradigms revealed distinct power dynamics in seizure frequencies.

Conclusions

This study facilitates a deeper understanding of seizure mechanisms using advanced technology.
The findings may contribute to identifying new therapeutic targets for epilepsy treatment.
The research emphasizes the importance of spatial and temporal analysis in understanding seizure activity.