Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

Anna E. Tseng; Aoife O'Connell; Aaron Benhamou; Shivraj Yabaji; Igor Kramnik; Nicholas A. Crossland; Emily Speranza

doi:10.3791/67853

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

迭代漂白通过在核心设施中使用染色自动化来扩展多样性

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04:52 min

August 6, 2025

DOI: 10.3791/67853-v

Anna E. Tseng^1,2, Aoife O'Connell¹, Aaron Benhamou^1,3, Shivraj Yabaji^1,2, Igor Kramnik^1,2, Nicholas A. Crossland^1,2,3, Emily Speranza⁴

¹National Emerging Infectious Diseases Laboratories (NEIDL),Boston University, ²Department of Pathology and Laboratory Medicine,Boston University Chobanian and Avedisian School of Medicine, ³Department of Virology, Immunology and Microbiology,Boston University Chobanian and Avedisian School of Medicine, ⁴Florida Research and Innovation Center,Cleveland Clinic Foundation

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在这里，我们描述了迭代漂白扩展多重性（IBEX）方法的应用，该方法利用市售的自动化组织染色机和基于酪胺信号放大的试剂的优化考虑因素。

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