基于DNA条形码的多重免疫荧光成像分析基因重编程小鼠黑色素瘤的福尔马林固定石蜡包埋样本

1.6K 次观看

09:58 分钟

2025年6月6日

10.3791/68124-v

2025年6月6日

1.6K 次观看

Sachin S. Surwase^*¹^,²^,³ , Xin Ming M. Zhou^*³^,⁴ , Kathryn M. Luly¹^,²^,³ , Qingfeng Zhu⁵^,⁶^,⁷ , Niki Talebian⁶ , Robert A. Anders⁵^,⁶^,⁷^,⁸^,⁹ , Jordan J. Green¹^,²^,³^,⁵^,⁸^,⁹^,¹⁰^,¹¹^,¹² , Stephany Y. Tzeng¹^,²^,³ , Joel C. Sunshine¹^,³^,⁴^,⁵^,⁶^,⁸^,⁹

¹Department of Biomedical Engineering, Johns Hopkins University, ²Translational Tissue Engineering Center, Johns Hopkins University, ³Johns Hopkins Translational ImmunoEngineering Center, Johns Hopkins University, ⁴Department of Dermatology, Johns Hopkins University, ⁵Department of Oncology, Johns Hopkins University, ⁶Department of Pathology, Johns Hopkins University, ⁷Convergence Institute, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University School of Medicine, ⁸Bloomberg Kimmel Institute for Cancer Immunotherapy, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, ⁹Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, ¹⁰Institute for Nanobiotechnology, Johns Hopkins University, ¹¹Departments of Neurosurgery and Ophthalmology, Johns Hopkins University School of Medicine, ¹²Departments of Materials Science & Engineering and Chemical & Biomolecular Engineering, Johns Hopkins University

^* These authors contributed equally

本方案提供了针对基于DNA条形码成像的小鼠FFPE黑色素瘤组织，设计多重免疫荧光抗体组合的逐步操作指南。我们还描述了使用开源工具进行图像分析的流程，以获得关于小鼠黑色素瘤肿瘤免疫微环境的空间蛋白质组学见解。

探索更多视频

DNA条形码成像

空间蛋白质组学

抗体面板设计

组织微环境

图像分析流程

细胞表型分析

肿瘤微环境

Chapters in this video

0:00

Introduction

1:21

Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Staining and Imaging for Multiplex Analysis

4:37

Digital Pathology Analysis and Proteomics Data Normalization

8:34

Results

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