Quantitative Analyse der Kletter Defekte in einem Drosophila Modell von neurodegenerativen Erkrankungen
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Summary
Abstract
Introduction
Protocol
Representative Results
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
| Drosophila stocks | The stocks are selected depending on the experiments. The temperature and humidity in the room and in the incubator must be controled and consistent to avoid flies being too staticky or too wet. | ||
| Video camera | Any digital camcorder will do. Make sure they can focus on close object. | ||
| Graduated cylinder | Kimble | 20028W | Different models of graduated cylinder may have different diameter. It is therefore imporant to measure the height. |
| Computer | Any model will do. We used the computer to monitor the climbing of the flies and record the number of flies at each time point. |
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Cite This Article
Madabattula, S. T., Strautman, J. C., Bysice, A. M., O’Sullivan, J. A., Androschuk, A., Rosenfelt, C., Doucet, K., Rouleau, G., Bolduc, F. Quantitative Analysis of Climbing Defects in a Drosophila Model of Neurodegenerative Disorders. J. Vis. Exp. (100), e52741, doi:10.3791/52741 (2015).