Mouse Tissue Isolation
Mouse Tissue Isolation - Related Videos
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
2013
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
11:14
Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry
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Cited by 7 •
2011
Flow cytometry is a powerful tool allowing for the isolation and study of specific cell populations. This protocol describes steps for isolating LacZ-expressing cells from cochlear tissues from neonatal transgenic mice. Dissociated cochlear cells were labeled using fluorescent-conjugated substrates of β-galactosidase prior to separation via flow cytometry.
10:50
Isolation of Pericytes from Mouse Cortical Tissue Using FACS for Single-cell Sequencing
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Cited by 1 •
2026
To obtain mouse brain cortical pericytes meeting the demands of scRNA-seq, we present a critically optimized FACS protocol based on CD13⁺/CD31⁻ selection. Our key refinements significantly enhance cell viability and RNA integrity, enabling reliable transcriptomic profiling.
05:27
Isolation and Characterization of Exosomes Derived from Mouse Spleen Tissues
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Cited by 6 •
2024
Here, we present a protocol to isolate mouse spleen-derived exosomes using a combination of digestion with collagenase type I and ultracentrifugation.
07:09
Isolation of Adipose Tissue Immune Cells
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Cited by 75 •
2013
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
FAQs
Immediate handling helps preserve the biological state of the sample after collection. Proteins, enzymes, metabolites, and nucleic acids can require stable conditions to remain suitable for analysis, so delays or inconsistent treatment may affect measured results. Protecting these components improves the reliability of biochemical and molecular assays performed on the isolated tissue.
Careful removal of surrounding tissues helps ensure that the collected material represents the intended anatomical region rather than a mixture of neighboring tissues. This improves tissue definition and supports more specific interpretation of gene expression, enzyme activity, signaling, or metabolite measurements. The result is stronger comparability between samples and more meaningful tissue-specific conclusions.
The handling strategy should protect the molecular components relevant to the planned assay. Isolated tissue may provide proteins and enzymes for activity measurements, metabolites for biochemical profiling, or nucleic acids for molecular studies. Preserving these components allows investigators to connect tissue structure with gene expression, signaling pathways, and other tissue-specific biochemical changes.
A basic workflow includes anatomical dissection, identification of the defined tissue, careful separation from surrounding material, and immediate handling under conditions suited to sample preservation. Consistency across these stages matters because variation in collection or treatment can alter sample quality. A standardized workflow therefore supports comparable biochemical and molecular measurements across experimental samples.
Researchers improve consistency by collecting the same defined tissue, applying comparable anatomical dissection and removal procedures, and handling each sample promptly under preservation-focused conditions. Standardization reduces unwanted variation in tissue composition and molecular stability. It is especially important when comparing enzyme activity, gene expression, signaling pathways, or other biochemical measurements between experimental groups.
This approach is useful when investigators need to examine biochemical changes within a particular tissue rather than in an undifferentiated biological sample. It can support studies of normal physiology, disease mechanisms, drug responses, and interactions between tissues and their molecular environments. The resulting tissue-specific material enables comparisons between biological conditions and experimental groups.
Isolated tissues can support measurements of gene expression, enzyme activity, signaling pathways, and tissue-specific biochemical changes. These outcomes help connect an anatomical sample with molecular processes occurring in that tissue. In biochemistry, such measurements can clarify how physiological or disease-related conditions influence proteins, metabolites, nucleic acids, and other molecular features.