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Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry
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JoVE Journal Neuroscience
Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

DOI:
10.3791/3432-v

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11:14 min

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December 23, 2011

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, , ,
1Department of Otolaryngology-Head and Neck Surgery,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 01:03Microdissection of the Cochlea
  • 03:24Cell Dissociation
  • 06:57Flow Cytometry
  • 09:15Representative Results
  • 10:52Conclusion

Summary

Automatic Translation

Automatic Translation

Flow cytometry is a powerful tool allowing for the isolation and study of specific cell populations. This protocol describes steps for isolating LacZ-expressing cells from cochlear tissues from neonatal transgenic mice. Dissociated cochlear cells were labeled using fluorescent-conjugated substrates of β-galactosidase prior to separation via flow cytometry.

Tags

Isolating LacZ-expressing CellsFlow CytometryRare Cell PopulationsStem/progenitor CellsTransgenic Reporter Mouse ModelsFluorescence-conjugated ProbesLacZ-positive CellsNeonatal CochleaeSensorineural Hearing LossCochlear Sensory Hair CellsRegenerative PotentialMurine Auditory And Vestibular OrgansStem/progenitor Cell CharacteristicsWnt Signaling PathwayAxin2-expressing CellsBehavior Of Wnt-responsive CellsAxin2-LacZ Reporter Mice

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