Cell-surface Interactions
Cell-surface Interactions - Related Videos
10:13
Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells
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Cited by 11 •
2010
A novel approach that allows the high-resolution analysis of cancer cell interactions with exogenous hyaluronic acid (HA) is described. Patterned surfaces are fabricated by combining carbodiimide chemistry and microcontact printing.
12:30
Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
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Cited by 56 •
2012
AVEXIS is a high throughput protein interaction assay developed to systematically screen for novel extracellular receptor-ligand pairs involved in cellular recognition processes. It is specifically designed to detect transient protein interactions that are difficult to identify using other high throughput approaches.
11:14
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
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Cited by 8 •
2017
A microfluidic chip was fabricated to produce pairs of gold dots for tandem bubble generation and fibronectin-coated islands for single-cell patterning nearby. The resultant flow field was characterized by particle image velocimetry and was employed to study various bioeffects, including cell membrane poration, membrane deformation, and intracellular calcium response.
05:21
Surface Plasmon Resonance to Study Biomolecular Interactions Using a Sensor Chip
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2025
This video describes the surface plasmon resonance, or SPR, technique for studying the interaction between two molecules using a sensor chip. The change in resonance angle and the intensity of the reflected light are detected as a function of the association and dissociation of two molecules, and this provides information about the interactions between the two molecules.
07:42
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
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Cited by 7 •
2010
This video shows experiments with subsequent analysis of protein-protein interactions by the use of micro-patterned surfaces. The approach offers the possibility to detect protein interactions in living cells and combines high throughput capabilities with the possibility to extract quantitative information.
FAQs
Integrins and other membrane receptors bind extracellular matrix components, engineered surfaces, or neighboring-cell molecules at the plasma membrane. These binding events transmit chemical and mechanical information into the cell, influencing adhesion, spreading, migration, and differentiation. In bioengineering, controlling which receptors engage with a surface helps researchers guide cell behavior rather than treating attachment as a purely passive event.
Surface chemistry, stiffness, and ligand presentation are central variables. Surface chemistry affects which molecular groups are available for contact, while stiffness supplies mechanical information that cells can sense. Ligand presentation determines how relevant binding sites are arranged and accessed. Adjusting these properties allows engineered materials to produce different patterns of adhesion, spreading, migration, or differentiation.
Cell-cell contacts involve recognition and binding between neighboring plasma membranes, whereas cell-material interactions occur when membrane receptors engage extracellular matrix components or ligands presented by an engineered surface. Both can trigger intracellular signaling, but their molecular context differs. Distinguishing these interaction types helps bioengineers design culture platforms and scaffolds that reproduce selected aspects of a cellular environment.
A typical design process begins by selecting a material or culture surface, then tuning its chemistry, stiffness, and ligand presentation to provide the desired cellular cues. Researchers place cells in contact with the engineered environment and examine responses such as adhesion, spreading, migration, or differentiation. The surface can then be refined to improve compatibility and more precisely control behavior.
They are especially important when a scaffold must support attachment and guide subsequent cell behavior. Receptor binding to scaffold-associated extracellular matrix components can influence whether cells adhere, spread, migrate, or differentiate. By tuning the scaffold’s surface chemistry, stiffness, and ligand presentation, researchers can create environments intended to support regenerative therapies rather than merely provide structural support.
Engineered culture platforms can reveal how defined surface cues affect adhesion, spreading, migration, and differentiation. These measurements help researchers connect material properties with cellular responses and assess biocompatibility. The same approach supports in vitro disease models, where controlling the cell environment can make it easier to study how cells respond to engineered or tissue-like conditions.