Ficoll 70
Ficoll 70 - Related Videos
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
0 Views •
Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
09:16
Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
0 Views •
Cited by 1 •
2019
Here, we present a protocol to test glomerular permeability in mice using a highly sensitive, nonradioactive tracer. This method allows repetitive urine analyses with small urine volumes.
14:52
Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy
0 Views •
2018
Here, a protocol involving re-arterialized rat partial liver transplantation is presented. Specifically, 70% liver was resected in vivo by using an updated technique of vessel-oriented hepatectomy. The hepatic artery was reconstructed in an end-to-side manner. The cuff technique was modified to shorten the anastomosis time of the infrahepatic vena cava.
07:14
Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70
0 Views •
Cited by 1 •
2024
This protocol demonstrates the chaperone activity of heat shock protein 70 (Hsp70). E. coli dnaK756 cells serve as a model for the assay as they harbor a native, functionally impaired Hsp70, making them susceptible to heat stress. The heterologous introduction of functional Hsp70 rescues the growth deficiency of the cells.
08:38
Electrospray Deposition of Uniform Thickness Ge23Sb7S70 and As40S60 Chalcogenide Glass Films
0 Views •
Cited by 8 •
2016
A method of uniform thickness solution-derived chalcogenide glass film deposition is demonstrated using computer numerical controlled motion of a single-nozzle electrospray.
FAQs
During density-gradient centrifugation, the concentration of Ficoll 70 establishes a medium with a defined density. Cells or other particles move through that medium until their migration reflects buoyant-density differences. This enables separation based on a physical property of the sample and can help distinguish components with different buoyant densities in a mixed biological preparation.
Ficoll 70 concentration is the main experimental variable for tuning the surrounding solution. Adjusting it changes the medium’s density and viscosity, while also altering the extent of macromolecular crowding. In separation experiments, this helps establish conditions governing particle migration; in biochemical models, it helps create a chosen solution environment.
Excluded-volume effects make Ficoll 70 valuable in crowded biochemical models. The polymer occupies solution space, so biomolecules experience a reduced accessible volume and may have higher effective concentrations than in an uncrowded solution. Bioengineers can therefore use it to examine reactions or molecular assembly under conditions designed to represent crowded biological environments.
Density-gradient centrifugation and macromolecular crowding use the same polymer for different experimental purposes. The first exploits a Ficoll 70 medium to resolve cells or particles by buoyant density, whereas the second uses excluded-volume behavior to modify effective biomolecule concentrations. Keeping these goals separate helps researchers target physical separation or biochemical-environment modeling.
To use Ficoll 70 for density-based separation, a researcher prepares a medium with an appropriate polymer concentration, places the biological sample in that defined environment, and applies centrifugation. Cells or particles then migrate according to buoyant density. Their resulting distribution provides the basis for separating or analyzing sample components within the gradient.
Ficoll 70 is useful when a cell-processing workflow requires populations to be distinguished through density-gradient centrifugation. Because the medium creates defined physical conditions, the approach can support separation of cells or particles according to buoyant density. This makes the polymer relevant to bioengineering studies that handle heterogeneous biological samples.
Researchers can add Ficoll 70 to in vitro systems when they want to study biochemical reactions or molecular assembly under crowded conditions. Its excluded-volume effects increase effective biomolecule concentrations, allowing experiments to test how a more physiologically relevant solution environment influences the system. This use complements, rather than replaces, density-gradient applications.
Beyond cell separation, Ficoll 70 supports biomimetic crowded environments and tissue engineering research. In these settings, researchers can control solution density, viscosity, or crowding to build experimental conditions that approximate important physical features of biological systems. Such models support the design of physiologically relevant experimental systems for controlled bioengineering studies.