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Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
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Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

DOI:
10.3791/59064-v

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09:16 min

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August 09, 2019

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, , , , ,
1Department of Nephrology, Medical Faculty,Heinrich-Heine-University

Chapters

  • 00:04Title
  • 00:28Preparation Phase Urinary Catheter
  • 01:43Preparation Phase – Central Venous Catheter
  • 04:16Blood Pressure Measurement and Equilibration Phase
  • 05:51Experimental Phase
  • 07:10Results: Fluorescence Scan, Glomerular Permeability and Blood Pressure
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to test glomerular permeability in mice using a highly sensitive, nonradioactive tracer. This method allows repetitive urine analyses with small urine volumes.

Tags

Keywords: Glomerular PermeabilityFluorescein Isothiocyanate-polysucrose 70NephrologyKidney DiseaseAnesthesiaUrinary CatheterJugular VeinLigatureFluorescent Tracer

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